Biyologlar - Biyolojiye Gerçekçi Yaklaşım

Medium and Buffer composition :

LB medium :
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10 g tryptone
05 g yeast extract
10 g NaCl
Add distilled water to a final volume of 1L and then autocalve

TE buffer ;
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10 mM Tris-HCl (pH 8.0)
1 mM EDTA (pH 8.0)

Lysis buffer :
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93.4 ml TE buffer
06 ml of 10% SDS
600 μl of proteinase K (20 mg/ml)

Kaynak: He, F. (2011). E. coli Genomic DNA Extraction. Bio-protocol Bio101: e97.